Quantitative sperm characteristics of Tankwa goats with special reference to hyperactivated motility

Computer-assisted semen analysis (CASA) is an automated and objective method of evaluating structural (e.g. morphology) functional sperm parameters motility hyperactivation). Sperm hyperactivation essential for successful fertilization thus important aspect in determining the fertility potential a male. In current study, CASA was used standard comparison ability phosphate buffered saline (PBS), BO wash (10 mM caffeine), 4% lignocaine, 5 procaine hydrochloride to induce Tankwa goat spermatozoa. Twenty-nine ejaculates were collected from randomly selected male goats by electroejaculation. Although none four media affected percentage total motility, lignocaine caused significant decrease (P >0.05) progressive motility. Exposure resulted increase swimming speed ≤0.05) star-spin tracks, which are typical hyperactivation. Using PBS data individually spermatozoa, receiver operator characteristic curves constructed distinguish kinematic employed as cut-off values did not (0.1 + 0.2% 0.04  respectively), while induced little (3.4 3.0%) had highest (25.3 13.6%). The large variation (0–54.5%) may reflect inter-individual differences quality among these males. This study indicated most promising hyperactivation-inducing medium spermatozoa should be considered similar assessments other animal species
 Keywords: computer-aided analysis, hydrochloride, kinematics, morphometry,